THE ACTION OF PHOSPHOLIPASES ON SUCCINATE OXIDASE AND CYTOCHROME OXIDASE
نویسندگان
چکیده
منابع مشابه
Studies on cytochrome oxidase. IV. The cytochrome oxidase activity.
where the activity is determined by measuring the rate of oxidation of reduced cytochrome c. Smith (6, 7), Conrad (8, 9), Wainio (10-13) et ~2. have extensively investigated the effect of various factors on the cytochrome oxidase activity measured by System 2 with soluble and insoluble preparations of cytochrome oxidase, whereas among those (14-30) who have employed System 1, Slater (5), using ...
متن کاملStudies on Cytochrome Oxidase
Ever since cytochrome oxidase was first solubilized from heart muscle preparations by Yakushiji and Okunuki (1) and Straub (2) in 1941, the further purification of this enzyme has been the subject of studies by many workers, using extraction with bile salts (3-14), or digestive enzymes such as trypsin (6, 7) and snake venom (15), fractionation with ammonium sulfate (5-14), chromatography (9, 12...
متن کاملStudies on cytochrome oxidase. V. Photochemical action spectra of CO inhibition of reconstituted cytochrome and lactate oxidase systems.
In 1929, Warburg and Negelein (1, 2) measured the action spectrum of the photoreversible inhibition of respiration by CO, The photochemical action spectra obtained with To&a utilis and Acetobactor pasteurianum exhibited absorption maxima at 280, 430, 545, and 590 rnp. Since these spectra resembled those obtained with heme compounds, Warburg and Negelein assumed that the respiratory enzyme invol...
متن کاملStudies on Cytochrome Oxidase. Vi. Kinetics of the Aerobic Oxidation of Ferrocytochrome C by Cytochrome Oxidase.
The rate of reaction can be determined by measuring the rate of either oxygen consumption or ferrocytochrome c oxidation. When the rate of oxygen consumption is measured either manometrically or polarographically, a mixture of a limited amount of cytochrome c and an excess of reducing agent, instead of excess ferrocytochrome c, is used as an electron donor system. In this case the true electron...
متن کاملThe Effect of Deoxycholate on Cytochrome Oxidase.
Sodium deoxycholate and sodium cholate have been widely used to solubiliie mitochondrial enzymes ever since their use by Keilin and Hartree for clarifying heart muscle preparations (l), by Hopkins, Lutwak-Mann, and Morgan (2) for extracting succinic dehydrogenase, by von Euler and Hellstrom (3) for preparing cytochrome b and cytochrome oxidase (cytochrome c:Os oxidoreductase, EC 1.9.3.1), and b...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1954
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)65489-4